hyodysenteriae strains and can be used as a species-specific marker. hyodysenteriae reference strains from diverse geographical locations shows that this fragment is conserved among S. The presence of the 3-kb Sau3A DNA fragment in S. Neither the 1.1-kb nor the 0.75-kb DNA probe hybridized with Asp 700- or Sau3A-digested S. hyodysenteriae reference strain and the Australian isolate S. hyodysenteriae-specific DNA probes (0.75 and 1.1 kb of DNA), distinct hybridization patterns for each S. When the chromosomal DNA was digested with the restriction endonuclease Asp 700 and hybridized with two S. hyodysenteriae strains but was absent from Serpulina innocens strains. hyodysenteriae-specific DNA probe, a common 3-kb band was always detected in S. hyodysenteriae were digested with the restriction endonuclease Sau3A and hybridized with a 1.1-kb S. When chromosomal DNAs from selected strains (reference serotypes) of S. Identification and characterization of Serpulina hyodysenteriae by restriction enzyme analysis and Southern blot analysis.Ĭhromosomal DNA restriction enzyme analysis and Southern blot hybridization were used to characterize Serpulina hyodysenteriae strains. These results suggest chromosomal rearrangements are only of minor significance in the establishment of reproductive barriers for this species. The remaining karyotypes were alternative hybrid forms, with 2n varying from 42 to 46 and AN from 68 to 80. Of the 26 karyotypes, only 14% presented a parental-like configuration, and none had the combinations of 2n and AN expected for an F1 hybrid. Cytogenetic analysis recorded 26 different karyotypes exhibited by 50 individuals from the hybrid population. minutus from the parental populations (2n/AN = 42/74 and 48a/76) and their contact zone. Cytogenetic analysis and a survey of six microsatellite loci included 101 specimens of C. We describe variation at microsatellite loci and the chromosomal polymorphisms of a hybrid population, and hybridizing populations of Ctenomys minutus (the minor tuco-tuco) from the coastal plain of Rio Grande do Sul, southern Brazil. In the present study, it is evidence that PCR- Southern Hybridization analysis offered a reliable result due to its highly specific and sensitive properties in detecting non-halal additive such as plasma protein incorporation in surimi-based product.Ī hybrid zone of the genus Ctenomys: A case study in southern BrazilĬastilho, Camila S. The sensitivity of PCR- southern hybridization primers to detect each meat species was 0.1 ng. Of 17 brands, 3 (n = 3*3) brands were positive for chicken DNA, 1 (n = 1*3) brand was positive for goat DNA, and the remainder 13 brands (n = 13*3) have no DNA species detected. 17 (n = 17*3) different brands of surimi-based product were purchased randomly from Selangor local market in January 2013.

The primers used in this technique were targeted on mitochondria DNA (mtDNA) of cytochrome b (cyt b) gene sequence which able to differentiate 7 type (beef, chicken, duck, goat, buffalo, lamb and pork) of species on a single chip. Halal surveillance on halal ingredients incorporated in surimi based products were studied using polymerase chain reaction (PCR)- southern hybridization on chip analysis. Market surveillance on non-halal additives incorporated in surimi based products using polymerase chain reaction (PCR)- southern hybridization analysisĪravindran, S.

Southern hybridization analysis